This content is Free Access.
Kapitel
- 00:05Titel
- 00:56Removal of Cells with Ammonium Hydroxide Solution
- 03:21Sterile Preparation of Cell-derived Extracellular Matrix for Cell Functional Assays
- 05:01Isolation of Extracellular Matrix for SDS-PAGE, Immunoblot Analysis, or Proteomics
- 06:30Results: Isolation and Analysis of Cell-derived Extracellular Matrix
- 08:26Conclusion
The extracellular matrix plays a major role in defining the microenvironment of cells and in modulating cell behavior and phenotype. We describe a rapid method for the isolation of cell-derived extracellular matrix, which can be adapted to different scales for microscopic, biochemical, proteomic, or functional studies.
