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Fiche Fluorescence Microscopy Lumière de racines de plante qui pousse sur la surface d'un gel
JoVE Journal
Biologie
Author Produced
This content is Free Access.
JoVE Journal Biologie
Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel
DOI:

06:41 min

January 18, 2017

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Kapitel

  • 00:00Titel
  • 01:24Plant Cultivation (Arabidopsis thaliana seedlings)
  • 02:04Plant Sample Preparation
  • 03:28Microscope Setup
  • 05:18Ergebnisse
  • 06:00Conclusion

Summary

Automatische Übersetzung

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

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