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Geração de células dendríticas derivadas de monócitos humanos a partir de sangue inteiro
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Kapitel
- 00:05Titel
- 00:51Peripheral Blood Mononuclear Cell (PBMC) Enrichment
- 02:30Monocytes Anti-human CD14 Magnetic Particle Isolation
- 04:08Monocyte Interleukin (IL)-4 and Granulocyte Macrophage–Colony Stimulating Factor (GM-CSF) Stimulation
- 05:10Results: Representative Human Monocyte–Dendritic Cell Generation from Whole Blood
- 06:19Conclusion
Here, we demonstrate how monocytes are isolated by magnetic bead separation from peripheral blood mononuclear cells after density gradient centrifugation of human anti-coagulated blood. Following incubation for 5 days, human monocytes are differentiated into immature dendritic cells and are ready for experimental procedures in a non-clinical setting.
