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癌細胞スフェロイド培養における潜在的に新規薬剤標的を同定するためのスクリーニング機能ゲノミクスを利用
JoVE Journal
Krebsforschung
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JoVE Journal Krebsforschung
Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures
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癌細胞スフェロイド培養における潜在的に新規薬剤標的を同定するためのスクリーニング機能ゲノミクスを利用

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07:48 min

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December 26, 2016

DOI:

10.3791/54738-v

DOI:
10.3791/54738-v

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07:48 min
December 26, 2016

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1The Breast Cancer Now Toby Robins Research Centre, Division of Breast Cancer,The Institute of Cancer Research, 2Division of Molecular Pathology,The Institute of Cancer Research, 3Institute of Cancer Sciences,University of Manchester

Tags

Functional GenomicsSiRNA ScreenCancer Cell Spheroid3D CultureHypoxiaTargeted ScreenOncogenesTumor SuppressorsCell ViabilityApoptosisTransfectionCell CultureCell CountingHigh-throughput Screening

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Morrison, E., Wai, P., Leonidou, A., Bland, P., Khalique, S., Farnie, G., Daley, F., Peck, B., Natrajan, R. Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures. J. Vis. Exp. (118), e54738, doi:10.3791/54738 (2016).

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