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Retroviral Transduction av Helper T-celler som en genetisk strategi för att studera mekanismer som styr deras differentiering och funktion
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function
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Retroviral Transduction av Helper T-celler som en genetisk strategi för att studera mekanismer som styr deras differentiering och funktion

10,626 Views

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11:50 min

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November 04, 2016

DOI:

10.3791/54698-v

DOI:
10.3791/54698-v

•

11:50 min
November 04, 2016

•

5 Views
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1Department of Comparative Biomedical Sciences,The Royal Veterinary College, 2Institute of Physiology I, Cardiology & Vascular Medicine,Eberhard Karls University of Tuebingen, 3Department of Clinical Science and Services,The Royal Veterinary College

Tags

Retroviral TransductionHelper T CellsGenetic ApproachT Cell DifferentiationT Cell FunctionMicroRNA RegulationHEK293T CellsCalcium Phosphate Transfection

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Singh, Y., Garden, O. A., Lang, F., Cobb, B. S. Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function. J. Vis. Exp. (117), e54698, doi:10.3791/54698 (2016).

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