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使用非变性聚丙烯酰胺凝胶电泳人的MxA多亚基蛋白复合物的表征
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Biochemie
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JoVE Journal Biochemie
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
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使用非变性聚丙烯酰胺凝胶电泳人的MxA多亚基蛋白复合物的表征

9,976 Views

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08:55 min

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October 28, 2016

DOI:

10.3791/54683-v

DOI:
10.3791/54683-v

•

08:55 min
October 28, 2016

•

6 Views
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1Institute for Medical Virology,University of Zurich, 2Friedrich Miescher Institute for Biomedical Research

Summary

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This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.

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Cite this Article

Nigg, P. E., Pavlovic, J. Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis. J. Vis. Exp. (116), e54683, doi:10.3791/54683 (2016).

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