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मानव प्राथमिक सेल संस्कृतियों में circadian घड़ी जीन अभिव्यक्ति और हार्मोन के स्राव के समानांतर मापन
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November 11, 2016
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Summary
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Here, we describe settings to monitor in parallel circadian bioluminescence and the secretory activity of human islet cells and primary myotubes. For this, we employed lentiviral gene delivery of a luciferase core clock reporter, followed by in vitro synchronization and collection of outflow medium by continuous cell perifusion.
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Petrenko, V., Saini, C., Perrin, L., Dibner, C. Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures. J. Vis. Exp. (117), e54673, doi:10.3791/54673 (2016).
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