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Defining Substrate Specificities for Lipase and Phospholipase Candidates
Kapitel
- 00:05Titel
- 01:02Use Artificial Substrates for Optimizing Enzyme Activities of (Phospho)lipases
- 02:20In Vivo Identification of the Physiological Substrate of a Lipase
- 05:48In Vitro Identification of the Physiological Substrate of Diacylglycerol (DAG) Lipase
- 07:00Results: Predicted Phospholipase SMc01003 Acts on Artificial p-Nitrophenyl Acyl Ester and DAG
- 08:01Conclusion
Many predicted (phospho)lipases are poorly characterized with regard to their substrate specificities and physiological functions. Here we provide a protocol to optimize enzyme activities, search for natural substrates, and propose physiological functions for these enzymes.
