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عزل البروتينات الدهنية عالية الكثافة لغير الترميز الصغيرة RNA الكمي
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Biochemie
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JoVE Journal Biochemie
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification
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عزل البروتينات الدهنية عالية الكثافة لغير الترميز الصغيرة RNA الكمي

11,158 Views

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10:39 min

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November 28, 2016

DOI:

10.3791/54488-v

DOI:
10.3791/54488-v

•

10:39 min
November 28, 2016

•

12 Views
, , , , , ,
1Department of Medicine,Vanderbilt University School of Medicine, 2Center for Quantitative Sciences,Vanderbilt University School of Medicine, 3Department of Cancer Biology,Vanderbilt University School of Medicine

Summary

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This protocol describes the isolation and quantification of high-density lipoprotein small RNAs.

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HDLLipoproteinSmall RNANon-coding RNADensity Gradient UltracentrifugationBiomarkerCardiometabolic DiseaseHigh-throughput SequencingReal-time PCR

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Cite this Article

Michell, D. L., Allen, R. M., Landstreet, S. R., Zhao, S., Toth, C. L., Sheng, Q., Vickers, K. C. Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification. J. Vis. Exp. (117), e54488, doi:10.3791/54488 (2016).

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