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Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
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JoVE Journal Genetik
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

DOI:
10.3791/54446-v

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06:03 min

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September 20, 2016

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, ,
1Super-Bacteria Research Center,Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Microbiomics and Immunity Research Center,Korea Research Institute of Bioscience and Bioengineering (KRIBB), 3Department of Biotechnology,The Catholic University of Korea

Kapitel

  • 00:05Titel
  • 00:36Initial Cultivation and Stress Adaptation
  • 02:58Single-colony Isolation of the Stress-adapted Strain
  • 04:02Results: High Succinate Stress Adaptation
  • 05:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.

Tags

Adaptive Laboratory EvolutionMicroorganismChemostatE. ColiAerationAgitationOptical DensityHigh Stress MediumStressorGlycerolAgar Plate

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