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Immunology and Infection

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JoVE Journal Immunology and Infection
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
 

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Article DOI: 10.3791/54421-v • 06:57 min • September 18th, 2016
September 18th, 2016 •
1, 2, 3, 2
1APC Microbiome Institute, University College Cork, 2Division of Gastroenterology and Hepatology, University Hospital Basel, 3Institute of Microbiology and Biotechnology, University of Ulm

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Summary
Automatic Translation

In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).

Tags

Antigen-driven Colitis Model Antigen Presenting Cells T Cells CX3 CR1 Positive Mononuclear Phagocytes CD4 Positive T Cells Inflammatory Bowel Disease Colonic Lamina Propria Immune Cells' Interaction Pathogenicity T Cell Transfer Colitis Model Red Fluorescent Antigen-specific Naive CD4 T Cells Immunodeficient Host Green Fluorescent Protein Mononuclear Cells Blue Fluorescent Antigen-expressing Bacteria Disease Development Dissection Scissors Colon Tissue
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