Journal
/
Entwicklungsbiologie
/
Аффинное мечение Обнаружение эндогенного Рецепторы из эмбрионов данио рерио
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
Please note that all translations are automatically generated. Click here for the English version.

Аффинное мечение Обнаружение эндогенного Рецепторы из эмбрионов данио рерио

5,506 Views

•

08:39 min

•

August 31, 2016

DOI:

10.3791/54405-v

DOI:
10.3791/54405-v

•

08:39 min
August 31, 2016

•

2 Views
, ,
1Cell and Developmental Biology, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

Summary

Automatically generated

A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

Explore More Videos

Affinity LabelingReceptor DetectionZebrafish EmbryosEntwicklungsbiologieReceptor ExpressionBiochemical PropertiesAFLIPCell BiologyDeyolkingYolk RemovalLigand BindingProtein ExtractionBradford Assay

Read Article

Cite this Article

Molina-Villa, T., Mendoza, V., López-Casillas, F. Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos. J. Vis. Exp. (114), e54405, doi:10.3791/54405 (2016).

Download .ris file

Copy

Share Video

.

Copy