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Affinitatsmarkierung Nachweis endogener Receptors von Zebrafischembryonen
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
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Affinitatsmarkierung Nachweis endogener Receptors von Zebrafischembryonen

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08:39 min

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August 31, 2016

DOI:

10.3791/54405-v

DOI:
10.3791/54405-v

•

08:39 min
August 31, 2016

•

2 Views
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1Cell and Developmental Biology, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

Summary

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A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

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Cite this Article

Molina-Villa, T., Mendoza, V., López-Casillas, F. Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos. J. Vis. Exp. (114), e54405, doi:10.3791/54405 (2016).

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