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Entwicklungsbiologie
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Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

DOI:
10.3791/54405-v

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08:39 min

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August 31, 2016

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, ,
1Cell and Developmental Biology, Instituto de Fisiología Celular,Universidad Nacional Autónoma de México

Kapitel

  • 00:05Titel
  • 00:47Preparation of Embryo Protein Extract
  • 03:22Detection of Endogenous Receptor Protein
  • 05:45Results: AFLIP Detection of BG in Zebrafish Embryos
  • 07:08Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A novel technique for the detection of low abundance endogenous receptors present in zebrafish embryos is described. We have named it AFLIP because it consists of affinity labeling of the receptor by its ligand linked to immunoprecipitation.

Tags

Affinity LabelingReceptor DetectionZebrafish EmbryosEntwicklungsbiologieReceptor ExpressionBiochemical PropertiesAFLIPCell BiologyDeyolkingYolk RemovalLigand BindingProtein ExtractionBradford Assay

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