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SNARE-gemedieerde Fusie van Single proteoliposomen met Tethered Ondersteund dubbellagen in een microfluïdische Flow Cell gecontroleerd door gepolariseerde TIRF Microscopie
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SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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SNARE-gemedieerde Fusie van Single proteoliposomen met Tethered Ondersteund dubbellagen in een microfluïdische Flow Cell gecontroleerd door gepolariseerde TIRF Microscopie

10,723 Views

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10:58 min

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August 24, 2016

DOI:

10.3791/54349-v

DOI:
10.3791/54349-v

•

10:58 min
August 24, 2016

•

10 Views
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1Department of Cellular and Molecular Physiology,Yale University School of Medicine, 2Nanobiology Institute,Yale University, 3Department of Molecular Biophysics and Biochemistry,Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales,Centre National de la Recherche Scientifique (CNRS)

Tags

SNARE-mediatedProteoliposomesSupported BilayersMicrofluidic Flow CellPolarized TIRF MicroscopyMembrane FusionNeurotransmitter ReleaseHormone ReleaseVesicle DockingFusion PoreExocytosisEnvelope VirusesPDMSPhotolithographyMicrofabricationProtein PurificationImaging

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Nikolaus, J., Karatekin, E. SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy. J. Vis. Exp. (114), e54349, doi:10.3791/54349 (2016).

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