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单脂蛋白的SNARE介导融合在一个微流控流细胞拴支持双分子层通过偏光显微镜TIRF监控
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JoVE Journal Neurowissenschaften
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
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单脂蛋白的SNARE介导融合在一个微流控流细胞拴支持双分子层通过偏光显微镜TIRF监控

10,723 Views

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10:58 min

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August 24, 2016

DOI:

10.3791/54349-v

DOI:
10.3791/54349-v

•

10:58 min
August 24, 2016

•

10 Views
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1Department of Cellular and Molecular Physiology,Yale University School of Medicine, 2Nanobiology Institute,Yale University, 3Department of Molecular Biophysics and Biochemistry,Yale University, 4Laboratoire de Neurophotonique, Université Paris Descartes, Faculté des Sciences Fondamentales et Biomédicales,Centre National de la Recherche Scientifique (CNRS)

Tags

SNARE-mediatedProteoliposomesSupported BilayersMicrofluidic Flow CellPolarized TIRF MicroscopyMembrane FusionNeurotransmitter ReleaseHormone ReleaseVesicle DockingFusion PoreExocytosisEnvelope VirusesPDMSPhotolithographyMicrofabricationProtein PurificationImaging

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Cite this Article

Nikolaus, J., Karatekin, E. SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy. J. Vis. Exp. (114), e54349, doi:10.3791/54349 (2016).

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