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Journal
/
Entwicklungsbiologie
/
単離およびヒト胎盤組織由来の間葉系幹/間質細胞の増殖
JoVE Journal
Entwicklungsbiologie
Author Produced
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue
Please note that all translations are automatically generated.
Click here for the English version.
単離およびヒト胎盤組織由来の間葉系幹/間質細胞の増殖
DOI:
10.3791/54204-v
•
12:40 min
•
June 06, 2016
•
Rebecca A. Pelekanos
,
Varda S. Sardesai
,
Kathryn Futrega
,
William B. Lott
,
Michael Kuhn
,
Michael R. Doran
3
1
UQ Centre for Clinical Research
,
The University of Queensland
,
2
Translational Research Institute
,
Queensland University of Technology
,
3
Translational Research Institute
,
Mater Medical Research – University of Queensland
Kapitel
00:00
Titel
01:00
Methodologies
02:20
Introduction
03:12
Tissues to be Harvested
03:53
Preparation of the Placenta and the Placental Anatomy
04:40
Dissection of the Decidua (D) Tissue
05:40
Dissection of the Chorionic Plate (CP) Tissue and Dissection of the Chorionic Villi (CV) Tissue
06:36
Mincing and Enzymatic Digestion of the Placental Tissue
07:25
Collection of Mononuclear Cells and Plating into Flasks
09:33
Post Isolation – Cell Growth and Expansion
09:57
Representative Results: Culture MSC and Assay Cells for MSC Characteristics
12:07
Conclusion
Summary
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Automatische Übersetzung
ここで我々が分離し、これらの組織から間葉系幹/間質細胞(MSC)の拡大が続く人間の満期胎盤から胎児と母体組織の切開のための方法を記載しています。
Tags
Mesenchymal Stem Cells
MSC Isolation
Cell Culture
Fetal-derived Cells
Maternal Cells
Decidua
Chorionic Villi
Chorionic Plate
Article
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