Journal
/
Chemie
/
תיוג ספין בימוי אתר ולימודי ספקטרוסקופיות EPR של pentameric ליגנד מגודרת יון ערוצים
JoVE Journal
Chemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Chemie
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Please note that all translations are automatically generated. Click here for the English version.

תיוג ספין בימוי אתר ולימודי ספקטרוסקופיות EPR של pentameric ליגנד מגודרת יון ערוצים

10,512 Views

•

11:19 min

•

July 04, 2016

DOI:

10.3791/54127-v

DOI:
10.3791/54127-v

•

11:19 min
July 04, 2016

•

1 Views
, ,
1Department of Physiology and Biophysics,Case Western Reserve University, 2School of Medicine,Case Western Reserve University

Summary

Automatically generated

This article describes methods for site-directed spin labeling and reconstitution of pentameric ligand-gated channels for Electron Paramagnetic Resonance studies. This protocol can be adapted for any membrane protein. The reconstitution method described here can also be used for patch-clamp measurements of macroscopic and single-channel currents in a defined lipid system.

Explore More Videos

Site Directed Spin LabelingEPR SpectroscopyPentameric Ligand-gated Ion ChannelsMembrane Protein DynamicsGLICSite-directed MutagenesisProtein PurificationCell LysisMembrane Protein Reconstitution

Read Article

Cite this Article

Basak, S., Chatterjee, S., Chakrapani, S. Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels. J. Vis. Exp. (113), e54127, doi:10.3791/54127 (2016).

Download .ris file

Copy

Share Video

.

Copy