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Site-Directed Spin Labeling und EPR-spektroskopischen Untersuchungen von pentamerem liganden-gesteuerten Ionenkanäle
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JoVE Journal Chemie
Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Site-Directed Spin Labeling und EPR-spektroskopischen Untersuchungen von pentamerem liganden-gesteuerten Ionenkanäle

10,511 Views

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11:19 min

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July 04, 2016

DOI:

10.3791/54127-v

DOI:
10.3791/54127-v

•

11:19 min
July 04, 2016

•

1 Views
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1Department of Physiology and Biophysics,Case Western Reserve University, 2School of Medicine,Case Western Reserve University

Summary

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This article describes methods for site-directed spin labeling and reconstitution of pentameric ligand-gated channels for Electron Paramagnetic Resonance studies. This protocol can be adapted for any membrane protein. The reconstitution method described here can also be used for patch-clamp measurements of macroscopic and single-channel currents in a defined lipid system.

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Cite this Article

Basak, S., Chatterjee, S., Chakrapani, S. Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels. J. Vis. Exp. (113), e54127, doi:10.3791/54127 (2016).

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