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同基因小鼠黑色素瘤细胞注射，以确定其在肺转移潜能
JoVE Journal
Medizin
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JoVE Journal Medizin
Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs
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同基因小鼠黑色素瘤细胞注射，以确定其在肺转移潜能

17,231 Views

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07:31 min

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May 24, 2016

DOI:

10.3791/54039-v

DOI:
10.3791/54039-v

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07:31 min
May 24, 2016

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4 Views
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1Brain Tumor Center & Neuro-Oncology Unit, Department of Neurology,Beth Israel Deaconess Medical Center & Harvard Medical School, 2Division of Cancer Genetics, Department of Medicine,Beth Israel Deaconess Medical Center & Harvard Medical School

Summary

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Metastasis plays a profound role in the virulence of cancer, accounting for an estimated 90% of deaths. We report a protocol for a metastatic melanoma model in mice that is useful for determining the efficacy of therapeutic agents against this clinical phenomenon.

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Timmons, J. J., Cohessy, S., Wong, E. T. Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs. J. Vis. Exp. (111), e54039, doi:10.3791/54039 (2016).

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