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心外膜细胞小鼠胚胎心脏的体外培养
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JoVE Journal Ingenieurwesen
In Vitro Culture of Epicardial Cells From Mouse Embryonic Heart
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心外膜细胞小鼠胚胎心脏的体外培养

DOI:
10.3791/53993-v

•

06:31 min

•

April 27, 2016

•

, , , ,
1Program in Cardiovascular and Metabolic Disorders,Duke-NUS Graduate Medical School, 2National Heart Research Institute Singapore,National Heart Centre Singapore, 3The Hatter Cardiovascular Insititute,University College London

Kapitel

  • 00:05Titel
  • 00:50Embryonic Ventricle Retrieval
  • 02:38Epicardial Explant Culture
  • 04:22Results: Representative Epicardial Cell Differentiation and Migration
  • 05:56Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The epicardium is an essential source of multipotent cardiovascular progenitor cells and paracrine factors that are required for cardiovascular development and regeneration. We describe here a method to culture mouse embryonic epicardial cells.

Tags

Epicardial CellsEmbryonic HeartIn Vitro CultureVentriclesCardiac DevelopmentCardiovascularCell BiologyCell CultureExplant CultureDissectionMouse Embryo

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