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1Center for Cancer Pharmacology,University of Pennsylvania, 2Center for Excellence in Environmental Toxicology,University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery,University of Pennsylvania, 5A.J. Drexel Autism Institute,Drexel University
Kapitel
- 00:05Titel
- 00:38Preparation of Buffers and 100X Stock Solutions
- 01:35Platelet Isolation and Performing an Experiment
- 02:49Quenching and CoA Extraction
- 04:30Results: Analysis of Human Platelets to Study Mitochondrial Metabolism
- 05:21Conclusion
Her viser vi isolerte humane blodplater kan anvendes som en rullestol ex vivo modell for å studere metabolske tilpasninger som reaksjon på det komplekse I-inhibitor rotenon. Denne tilnærmingen benytter isotopisk sporing og relativ kvantifisering ved væske-kromatografi-massespektrometri og kan brukes på en rekke forskjellige studiedesign.
