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1Center for Cancer Pharmacology,University of Pennsylvania, 2Center for Excellence in Environmental Toxicology,University of Pennsylvania, 3Penn SRP and Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania, 4Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery,University of Pennsylvania, 5A.J. Drexel Autism Institute,Drexel University
Kapitel
- 00:05Titel
- 00:38Preparation of Buffers and 100X Stock Solutions
- 01:35Platelet Isolation and Performing an Experiment
- 02:49Quenching and CoA Extraction
- 04:30Results: Analysis of Human Platelets to Study Mitochondrial Metabolism
- 05:21Conclusion
Qui mostriamo isolato piastrine umane possono essere utilizzati come accessibili ex vivo modello per studiare adattamenti metabolici in risposta al complesso I inibitore rotenone. Questo approccio impiega tracing isotopica e relativa quantificazione dal liquido spettrometria cromatografia massa e può essere applicato ad una varietà di disegni di studio.
