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Индукция экспериментального аутоиммунного энцефаломиелита у мышей и оценка болезни зависимого распределения иммунных клеток в различных тканях
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Kapitel
- 00:05Titel
- 01:24Experimental Autoimmune Encephalitis (EAE) Induction and Flow Cytometric Immune Cell Analysis
- 05:35Results: Representative EAE-induced Spleen, Blood, LN and Spinal Cord Immune Cell and Cytokine mRNA Expression Analyses
- 07:37Conclusion
This manuscript describes the methods for induction and scoring of the experimental autoimmune encephalomyelitis (EAE) model, together with the assessment of immune cell distribution and mRNA cytokine levels in lymph nodes, spleen, blood and spinal cord using flow cytometry and quantitative PCR, respectively, at various disease phases.
