Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Kapitel
- 00:05Titel
- 00:53Labeling of the Reducing End (RE) Sugar Residue of W-sol AXs with 2-aminobenzamide (2AB)
- 02:11Generation of Xylo-oligosaccharides from 2 AB Labeled W-sol AXs
- 03:34Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry (MALDI-TOF MS)
- 04:48Electrospray Ionization Quadrupole Time-of-flight Mass Spectrometry (ESI-QTOF MS)
- 06:20ESI-MSn
- 07:30H1 Nuclear Magnetic Resonance (NMR) Spectroscopy
- 08:45Results: Reducing End (RE) and Internal Region Glycosyl Sequence(s) of Heteroxylans
- 10:34Conclusion
This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).
