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Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia
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JoVE Journal Neurowissenschaften
Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia

Sex Differences in Mouse Hippocampal Astrocytes after In-Vitro Ischemia

DOI:
10.3791/53695-v

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08:32 min

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October 25, 2016

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, , , , ,
1Waisman Center,University of Wisconsin, 2Department of Neurological Surgery,University of Wisconsin, 3Department of Pediatrics,University of Wisconsin

Kapitel

  • 00:05Titel
  • 00:42Hippocampal Dissection and Plating
  • 03:32Astrocyte Culture
  • 05:34Oxygen and Glucose Deprivation and Reoxygenation (OGD/REOX)
  • 06:21Results: Increased Protein Expression of HIF1α and Astrocytic Markers Following OGD/REOX
  • 07:46Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Astrocytes are one of the most important key players in the central nervous system (CNS). Here, we are reporting a practical method of sexed hippocampal astrocyte culture protocol in order to study the mechanisms underlying the astrocyte function in male and female neonate pups after in-vitro ischemia.

Tags

MouseHippocampalAstrocytesIn-vitroIschemiaSex DifferencesCell CultureOxygen And Glucose DeprivationReoxygenationHypoxic IschemiaNewbornBrain DissectionTrypsinAstrocyte Plating MediumCell Suspension

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