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CN-GELFrEE – Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis
1Departments of Chemistry and Molecular Biosciences, Chemistry of Life Processes Institute, Proteomics Center of Excellence, Robert H. Lurie Comprehensive Cancer Center,Northwestern University, 2Institute of Chemistry, Proteomics Unit,Federal University of Rio de Janeiro, 3Department of Cell Biology, Brazilian Center for Protein Research, Laboratory of Biochemistry and Protein Chemistry,University of Brasilia
Kapitel
- 00:05Titel
- 00:38Prepararing the Gradient Tube Gels
- 04:44Clear Native Gel-eluted Liquid Fraction Entrapment Electrophoresis (CN-GELFrEE) Setup
- 06:59CN-GELFrEE: Sample Loading to Analysis
- 09:13Results: Non-covalent Protein Assemblies from Mouse Heart
- 11:00Conclusion
This protocol describes how to prepare and perform clear native gel-eluted liquid fraction entrapment electrophoresis (CN-GELFrEE), a native separation technique for non-covalent biomolecular assemblies and proteins from heterogeneous samples that is compatible with various downstream protein analysis techniques.
