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Eşzamanlı İki foton<em> İn Vivo</em> Fare Retrosplenial kortekste sinaptik Girişlerin Görüntüleme ve postsinaptik Hedefler
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Kapitel
- 00:00Titel
- 01:27Surgery Preparation
- 03:37Cranial Window Surgery
- 08:30Virus Injection
- 10:43Cranial Window Implantation
- 13:15Imaging
- 15:12Ergebnisse
- 15:56Conclusions
This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.
