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半自动化的方法准备抗体鸡尾酒人外周血中免疫表型分析
JoVE Journal
Immunologie und Infektion
This content is Free Access.
JoVE Journal Immunologie und Infektion
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
DOI:

08:17 min

February 08, 2016

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Kapitel

  • 00:05Titel
  • 00:58Operating Procedure
  • 06:25Results: Low Variability in Semi-automated Whole Blood Immunophenotyping
  • 07:04Conclusion

Summary

Automatische Übersetzung

Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.

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