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Мышиные Кожные фибробластов Изоляция FACS
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Murine Dermal Fibroblast Isolation by FACS
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Мышиные Кожные фибробластов Изоляция FACS

20,034 Views

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06:04 min

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January 07, 2016

DOI:

10.3791/53430-v

DOI:
10.3791/53430-v

•

06:04 min
January 07, 2016

•

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1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Surgery, John A. Burns School of Medicine,University of Hawai’i

Tags

MurineDermalFibroblastIsolationFACSIn Vitro CulturePhenotypic ChangeEntwicklungsbiologieWound HealingFibroblast SubtypesScarringFibrosisPeritoneal FibroblastsAbdominal AdhesionsCollagenaseDMEMFBS100 Micron Strainer75 Micron Strainer

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Cite this Article

Walmsley, G. G., Maan, Z. N., Hu, M. S., Atashroo, D. A., Whittam, A. J., Duscher, D., Tevlin, R., Marecic, O., Lorenz, H. P., Gurtner, G. C., Longaker, M. T. Murine Dermal Fibroblast Isolation by FACS. J. Vis. Exp. (107), e53430, doi:10.3791/53430 (2016).

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