Bacterial attachment to host cells is a key step during host colonization and infection. This protocol describes the generation of polymer-coupled recombinant adhesins as biomimetic materials which allow analysis of the contribution of individual adhesins to these processes, independent of other bacterial factors.
Stones, D. H., Al-Saedi, F., Vaz, D., Perez-Soto, N., Krachler, A. M. Biomimetic Materials to Characterize Bacteria-host Interactions. J. Vis. Exp. (105), e53400, doi:10.3791/53400 (2015).