Journal
/
Biotechnik
/
상호 현미경을 사용하여 본래 세포의 표피 성장 인자 수용체의 화학 양론을 공부
JoVE Journal
Biotechnik
This content is Free Access.
JoVE Journal Biotechnik
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
Please note that all translations are automatically generated. Click here for the English version.

상호 현미경을 사용하여 본래 세포의 표피 성장 인자 수용체의 화학 양론을 공부

8,069 Views

•

09:16 min

•

September 11, 2015

DOI:

10.3791/53186-v

DOI:
10.3791/53186-v

•

09:16 min
September 11, 2015

•

1 Views
,
1INM-Leibniz Institute for New Materials, 2Department of Physics,University of Saarland

Summary

Automatically generated

This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.

Explore More Videos

Epidermal Growth Factor ReceptorEGFRCOS7 Fibroblast CellsCorrelative MicroscopyFluorescence MicroscopyEnvironmental Scanning Electron Microscopy (ESEM)Scanning Transmission Electron Microscopy (STEM)Quantum DotsReceptor StoichiometryReceptor DistributionReceptor Clustering

Read Article

Cite this Article

Peckys, D. B., de Jonge, N. Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy. J. Vis. Exp. (103), e53186, doi:10.3791/53186 (2015).

Download .ris file

Copy

Share Video

.

Copy