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Studiare la Stechiometria di Epidermal Growth Factor Receptor in cellule intatte con correlativa Microscopia
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
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Studiare la Stechiometria di Epidermal Growth Factor Receptor in cellule intatte con correlativa Microscopia

8,069 Views

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09:16 min

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September 11, 2015

DOI:

10.3791/53186-v

DOI:
10.3791/53186-v

•

09:16 min
September 11, 2015

•

1 Views
,
1INM-Leibniz Institute for New Materials, 2Department of Physics,University of Saarland

Summary

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This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.

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Peckys, D. B., de Jonge, N. Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy. J. Vis. Exp. (103), e53186, doi:10.3791/53186 (2015).

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