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Studieren der Stöchiometrie der Epidermal Growth Factor Receptor in intakten Zellen mit korrelative Mikroskopie
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Biotechnik
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JoVE Journal Biotechnik
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
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Studieren der Stöchiometrie der Epidermal Growth Factor Receptor in intakten Zellen mit korrelative Mikroskopie

8,069 Views

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09:16 min

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September 11, 2015

DOI:

10.3791/53186-v

DOI:
10.3791/53186-v

•

09:16 min
September 11, 2015

•

1 Views
,
1INM-Leibniz Institute for New Materials, 2Department of Physics,University of Saarland

Summary

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This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.

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Epidermal Growth Factor ReceptorEGFRCOS7 Fibroblast CellsCorrelative MicroscopyFluorescence MicroscopyEnvironmental Scanning Electron Microscopy (ESEM)Scanning Transmission Electron Microscopy (STEM)Quantum DotsReceptor StoichiometryReceptor DistributionReceptor Clustering

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Peckys, D. B., de Jonge, N. Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy. J. Vis. Exp. (103), e53186, doi:10.3791/53186 (2015).

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