Although mouse models are invaluable tools for bone tissue engineering, models of long bone defects are sparse. This need motivated development of the present protocol which uses a locking plate with four screws and a dedicated jig to perform and stabilize a reproducible, femoral, critical-size defect with low morbidity.
Manassero, M., Decambron, A., Huu Thong, B. T., Viateau, V., Bensidhoum, M., Petite, H. Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis. J. Vis. Exp. (116), e52940, doi:10.3791/52940 (2016).