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Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
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JoVE Journal Chemie
Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

DOI:
10.3791/52926-v

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10:27 min

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July 14, 2015

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, , , , ,
1The Molecular Foundry,E. O. Lawrence Berkeley National Laboratory, 2Institute of Chemical Sciences,Bahauddin Zakariya University, 3Department of Chemistry,University of the Balearic Islands, 4Beijing Key Lab of Bioprocess, College of Life Science and Technology,Beijing University of Chemical Technology

Kapitel

  • 00:05Titel
  • 02:01Functionalization of Sillica Capillaries for the Preparation of Monolithic Columns
  • 03:57Monolithic Capillary Column Preparation
  • 06:13Enrichment of Phosphopeptides
  • 08:00Results: Capillaries Enriched with Iron 3 Sites Show Increased Phosphopeptide Binding
  • 09:44Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

A procedure for the preparation of porous hybrid separation media composed of a macroporous polymer monolith internally coated by a high surface area microporous coordination polymer is presented.

Tags

Keywords: Porous Coordination PolymerMacroporous Polymer MonolithMetal-organic Polymer HybridPhosphopeptide EnrichmentAffinity ChromatographyCapillary ColumnMALDI-TOF MS

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