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Immunologie und Infektion
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In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

In Vitro and In Vivo Model to Study Bacterial Adhesion to the Vessel Wall Under Flow Conditions

DOI:
10.3791/52862-v

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10:24 min

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June 11, 2015

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, , , , ,
1Center for Molecular and Vascular Biology, Department of Cardiovascular Sciences,KU Leuven

Kapitel

  • 00:05Titel
  • 01:40S. aureus Preparation for In Vitro Perfusion and In Vivo Experiments
  • 03:00In Vitro Perfusion Experiments
  • 05:53In Vivo Mesentric Perfusion Model
  • 08:08Results: von Willebrand Factor is Essential for Shear Mediated Adhesion of S. aureus to Endothelial Cells and to the Subendothelial Matrix
  • 09:38Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

To study the interaction of bacteria with the blood vessels under shear stress, a flow chamber and an in vivo mesenteric intravital microscopy model are described that allow to dissect the bacterial and host factors contributing to vascular adhesion.

Tags

Bacterial AdhesionVessel WallFlow ConditionsEndovascular InfectionsInfective EndocarditisIn Vitro Flow ChamberIn Vivo Intravital MicroscopyExtracellular MatrixEndothelial CellsStaphylococcus AureusVon Willebrand FactorShear Stress

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