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噬菌体型组学:生理途径来表征新型病毒蛋白
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
DOI:

09:40 min

June 11, 2015

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Kapitel

  • 00:05Titel
  • 01:20Preparation of Multi-phenotype Assay Plates (MAPs)
  • 02:38MAP Optical Density Measurements
  • 03:27Continuous Culture Set-up
  • 05:07Running Continuous Cultures for Metabolomics
  • 07:15Representative Analysis of Unknown Phage Genes
  • 09:07Conclusion

Summary

Automatische Übersetzung

Here, we present phenomic approaches for the functional characterization of putative phage genes. Techniques include a developed assay capable of monitoring host anabolic metabolism, the Multi-phenotype Assay Plates (MAPs), in addition to the established method of metabolomics, capable of measuring effects to catabolic metabolism.

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