Journal
/
Entwicklungsbiologie
/
同軸ノズルを用いて多能性幹細胞のアルギン酸カプセル化
JoVE Journal
Entwicklungsbiologie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Entwicklungsbiologie
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
Please note that all translations are automatically generated. Click here for the English version.

同軸ノズルを用いて多能性幹細胞のアルギン酸カプセル化

12,373 Views

•

07:13 min

•

July 02, 2015

DOI:

10.3791/52835-v

DOI:
10.3791/52835-v

•

07:13 min
July 02, 2015

•

15 Views
,
1Center for International Research on Integrative Biomedical System, Institute of Industrial Science,The University of Tokyo

Summary

Automatically generated

We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

Explore More Videos

Alginate EncapsulationPluripotent Stem CellsCo-axial NozzleSuspension CultureAggregationShear StressCapsule SizeAlg-Ca CapsuleAlg-PLL CoatingStem Cell NicheMass Production

Read Article

Cite this Article

Horiguchi, I., Sakai, Y. Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle. J. Vis. Exp. (101), e52835, doi:10.3791/52835 (2015).

Download .ris file

Copy

Share Video

.

Copy