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多能干细胞的海藻酸钠封装使用同轴喷嘴
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
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多能干细胞的海藻酸钠封装使用同轴喷嘴

12,373 Views

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07:13 min

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July 02, 2015

DOI:

10.3791/52835-v

DOI:
10.3791/52835-v

•

07:13 min
July 02, 2015

•

15 Views
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1Center for International Research on Integrative Biomedical System, Institute of Industrial Science,The University of Tokyo

Summary

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We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

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Alginate EncapsulationPluripotent Stem CellsCo-axial NozzleSuspension CultureAggregationShear StressCapsule SizeAlg-Ca CapsuleAlg-PLL CoatingStem Cell NicheMass Production

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Cite this Article

Horiguchi, I., Sakai, Y. Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle. J. Vis. Exp. (101), e52835, doi:10.3791/52835 (2015).

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