Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Um Aquatic Microbial metaproteômica fluxo de trabalho: a partir de células para Tryptic Peptides adequado para análise baseada em espectrometria de massa em tandem
8,634 Views
•
08:09 min
•
September 15, 2015
DOI:
Summary
Automatically generated
This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.
Read Article
Cite this Article
Colatriano, D., Walsh, D. A. An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis. J. Vis. Exp. (103), e52827, doi:10.3791/52827 (2015).
Copy