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Kapitel
- 00:05Titel
- 03:18Aphid Rearing
- 04:14Making an Insect Electrode
- 05:58Wiring an Insect for Electrical Penetration Graph (EPG) Recordings
- 08:27EPG Recording
- 11:27Results: Electrical Response to Wounding in Central and Marginal Veins in Leaves of Arabidopsis thaliana
- 13:18Conclusion
Electrical Penetration Graph (EPG) is a well-established technique for studying the feeding behavior of stylet-bearing insects. Here we show a new application of EPG as a non-invasive tool for the acquisition of intracellular electrophysiology recordings of sieve elements (SEs), the cells that form the phloem vasculature in plants.
