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混合μCT-FMT成像和图像分析
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Hybrid µCT-FMT imaging and image analysis
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混合μCT-FMT成像和图像分析

12,817 Views

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13:45 min

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June 04, 2015

DOI:

10.3791/52770-v

DOI:
10.3791/52770-v

•

13:45 min
June 04, 2015

•

2 Views
, , , , , , ,
1Experimental Molecular Imaging,RWTH Aachen University, 2Institute for Biomedical Engineering – Biointerface Laboratory,RWTH Aachen University, 3Utrecht Institute for Pharmaceutical Sciences,Utrecht University

Summary

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We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.

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Hybrid ImagingMultimodal ImagingFluorescence-mediated Tomography (FMT)Micro-computed Tomography (μCT)Image RegistrationFluorescence ReconstructionQuantitative AnalysisOrgan SegmentationBiodistribution

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Cite this Article

Gremse, F., Doleschel, D., Zafarnia, S., Babler, A., Jahnen-Dechent, W., Lammers, T., Lederle, W., Kiessling, F. Hybrid µCT-FMT imaging and image analysis. J. Vis. Exp. (100), e52770, doi:10.3791/52770 (2015).

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