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Uzun süreli Kalsiyum Görüntüleme için Agaroz microchambers<em> Caenorhabditis elegans</em
JoVE Journal
Neurowissenschaften
This content is Free Access.
JoVE Journal Neurowissenschaften
Agarose Microchambers for Long-term Calcium Imaging of Caenorhabditis elegans
DOI:

07:51 min

June 24, 2015

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Kapitel

  • 00:05Titel
  • 01:00Instruments, Culture Media and Dishes
  • 01:58Preparation of Agarose Microchambers
  • 04:50Agarose Microfluidic Chambers Imaging
  • 05:37Calcium Imaging
  • 06:27Results: Long-term Calcium Imaging
  • 07:20Conclusion

Summary

Automatische Übersetzung

Imaging behavior and neural activity over long time scales without immobilization of the animal is a prerequisite to understand behavior. Agarose microfluidic chambers imaging (AMI) can be used to image neural activity and behavior for all life stages of Caenorhabditis elegans.

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