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앞으로 유전학 식별하는 대 식세포를 사용하여 화면<em> 톡소 포자충</em> 유전자 중요 저항이하는 IFN-γ 의존 세포 자치 내성
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
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앞으로 유전학 식별하는 대 식세포를 사용하여 화면<em> 톡소 포자충</em> 유전자 중요 저항이하는 IFN-γ 의존 세포 자치 내성

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11:21 min

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March 12, 2015

DOI:

10.3791/52556-v

DOI:
10.3791/52556-v

•

11:21 min
March 12, 2015

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2 Views
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1Department of Microbiology and Immunology,New York Medical College

Summary

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Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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Toxoplasma GondiiMacrophagesForward Genetics ScreenIFN-γCell Autonomous ImmunityParasite MutantsParasite GenesChronic InfectionTachyzoiteBradyzoite

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Walwyn, O., Skariah, S., Lynch, B., Kim, N., Ueda, Y., Vohora, N., Choe, J., Mordue, D. G. Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity. J. Vis. Exp. (97), e52556, doi:10.3791/52556 (2015).

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