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Forward Genetica schermi usando macrofagi per identificare<em> Toxoplasma gondii</em> geni importanti per la Resistenza di IFN-γ-Dependent cellulare Immunità autonoma
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
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Forward Genetica schermi usando macrofagi per identificare<em> Toxoplasma gondii</em> geni importanti per la Resistenza di IFN-γ-Dependent cellulare Immunità autonoma

10,923 Views

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11:21 min

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March 12, 2015

DOI:

10.3791/52556-v

DOI:
10.3791/52556-v

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11:21 min
March 12, 2015

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2 Views
, , , , , , ,
1Department of Microbiology and Immunology,New York Medical College

Summary

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Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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Walwyn, O., Skariah, S., Lynch, B., Kim, N., Ueda, Y., Vohora, N., Choe, J., Mordue, D. G. Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity. J. Vis. Exp. (97), e52556, doi:10.3791/52556 (2015).

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