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استهدفت DNA تحليل مثلأيشن من قبل الجيل المقبل من التسلسل
JoVE Journal
Biologie
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JoVE Journal Biologie
Targeted DNA Methylation Analysis by Next-generation Sequencing
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استهدفت DNA تحليل مثلأيشن من قبل الجيل المقبل من التسلسل

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08:38 min

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February 24, 2015

DOI:

10.3791/52488-v

DOI:
10.3791/52488-v

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08:38 min
February 24, 2015

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7 Views
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1Department of Physiology,University of Oklahoma College of Medicine, 2Department of Geriatric Medicine,University of Oklahoma College of Medicine

Summary

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Bisulfite amplicon sequencing (BSAS) is a method for quantifying cytosine methylation in targeted genomic regions of interest. This method uses bisulfite conversion paired with PCR amplification of target regions prior to next-generation sequencing to produce absolute quantitation of DNA methylation at a base-specific level.

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DNA MethylationEpigeneticsNext-generation SequencingBisulfite SequencingBisulfite Amplicon SequencingTargeted AnalysisGene ExpressionCytosine MethylationCpG IslandsQuantitative Analysis

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Cite this Article

Masser, D. R., Stanford, D. R., Freeman, W. M. Targeted DNA Methylation Analysis by Next-generation Sequencing. J. Vis. Exp. (96), e52488, doi:10.3791/52488 (2015).

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