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胚性齧歯類の脳から単離、プライマリ脳ドーパミンニューロンの文化と長期メンテナンス
Kapitel
- 00:05Titel
- 01:29Making Preparations
- 02:45Dissecting the Embryonic Ventral Midbrain
- 04:44Dissociating the Ventral Midbrain Cells
- 06:09Plating and Culturing the Ventral Midbrain Cells
- 07:49Results: Characterizing the Dopaminergic Neuron Cultures
- 08:21Conclusion
The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.
