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광 변환 한 후 형광 붕괴를 사용하여 생활 Zebrafish의 태아의 단백질 안정성을 측정 (FDAP)
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Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)
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광 변환 한 후 형광 붕괴를 사용하여 생활 Zebrafish의 태아의 단백질 안정성을 측정 (FDAP)

11,066 Views

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09:45 min

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January 28, 2015

DOI:

10.3791/52266-v

DOI:
10.3791/52266-v

•

09:45 min
January 28, 2015

•

5 Views
, , ,
1Department of Molecular and Cellular Biology,Harvard University, 2Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Tags

Protein StabilityFluorescence Decay After PhotoconversionFDAPProtein Clearance KineticsPhotoconvertible Fluorescent ProteinZebrafish EmbryosCompartmental AnalysisIntra- And Extracellular Half-livesSecreted Signaling ProteinsIn Vivo Protein Dynamics

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Rogers, K. W., Bläßle, A., Schier, A. F., Müller, P. Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP). J. Vis. Exp. (95), e52266, doi:10.3791/52266 (2015).

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