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High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies
JoVE Journal
Medizin
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JoVE Journal Medizin
High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

DOI:
10.3791/52242-v

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09:19 min

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January 04, 2015

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, , , , , ,
1Department of Chemical and Biological Engineering,Iowa State University, 2Department of Genetics, Development and Cell Biology,Iowa State University, 3Biology Program,Iowa State University

Kapitel

  • 00:05Titel
  • 01:45Substrate Preparation and Cell Plating
  • 03:35Time Lapse Imaging
  • 05:01Ki67 Cell Proliferation, Automated Imaging and Multi-wavelength Scoring Analysis
  • 07:00Results: Characterization of Engineered MSCs
  • 08:29Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This study describes an experimental platform to rapidly characterize engineered stem cells and their behaviors before their application in long-term in vivo transplant studies for nervous system rescue and repair.

Tags

Mesenchymal Stem CellsGenetic ModificationBrain-derived Neurotrophic FactorGlial Cell-derived Neurotrophic FactorHigh Content ScreeningCell AdhesionCell ProliferationCell MigrationCell ViabilityNeuroprotective Strategies

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